VG5Q (AGGF1) (NM_018046) Human Tagged Lenti ORF Clone

Cat# RC205994L4

Size : 10ug


VG5Q (AGGF1) (NM_018046) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC205994L4
Lenti ORF clone of Human angiogenic factor with G patch and FHA domains 1 (AGGF1), mGFP tagged
 
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VG5Q
Synonyms GPATC7; GPATCH7; HSU84971; HUS84971; VG5Q
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205994).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_018046
ORF Size 2142 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018046.3
RefSeq Size 4480 bp
RefSeq ORF 2145 bp
Locus ID 55109
UniProt ID Q8N302
Cytogenetics 5q13.3
Domains FHA, G-patch
Protein Families Secreted Protein
MW 80.8 kDa
Summary This gene encodes an angiogenic factor that promotes proliferation of endothelial cells. Mutations in this gene are associated with a susceptibility to Klippel-Trenaunay syndrome. Pseudogenes of this gene are found on chromosomes 3, 4, 10 and 16.provided by RefSeq, Sep 2010
SKU Description Size
RC205994 AGGF1 (Myc-DDK-tagged)-Human angiogenic factor with G patch and FHA domains 1 (AGGF1) 10 ug
RC205994L1 Lenti ORF clone of Human angiogenic factor with G patch and FHA domains 1 (AGGF1), Myc-DDK-tagged 10 ug
RC205994L2 Lenti ORF clone of Human angiogenic factor with G patch and FHA domains 1 (AGGF1), mGFP tagged 10 ug
RC205994L3 Lenti ORF clone of Human angiogenic factor with G patch and FHA domains 1 (AGGF1), Myc-DDK-tagged 10 ug
RG205994 AGGF1 (tGFP-tagged) - Human angiogenic factor with G patch and FHA domains 1 (AGGF1) 10 ug
SC127530 AGGF1 (untagged)-Human angiogenic factor with G patch and FHA domains 1 (AGGF1) 10 ug

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