WB / Antibody Stripping Buffer

WB Stripping Buffer is used for the recycle of deproteinized membrane in WB. In WB, after completing the conjugation of the primary and secondary antibodies as well as the later chemiluminescence, sometimes end user has to detect the proteins with stable expression level like Actin and Tubulin, etc. for reference, or test other proteins for comparison. But for this reagent, through dissociating the conjugation of primary, secondary antibodies and antigens, it removes primary and secondary antibodies from the membrane, then end user can reuse the same membrane to detect other proteins. Thus, compare with running a fresh SDS-PAGE gel, this reagent helps to eliminate the loading error and makes the experiment more comparable and accurate.
For excluding the frequently-used beta-Mercaptoethanol, this reagent is nontoxic, tasteless and harmless, can be used at room temperature. This reagent is enough for at least 5 times WB detections on the same membrane, and the whole detection can be completed within15-30 min.

Small volumes of WB Stripping Buffer (Antibody Stripping Buffer) buffer vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS355701 is a buffer targeting WB Stripping Buffer (Antibody Stripping Buffer). Also known as WB / Antibody Stripping Buffer.