Cyanine 5 (Cy5)-conjugated anti-guinea pig antibodies are far-red fluorescent immunoreagents designed for the sensitive detection of guinea pig antigens in fluorescence-based assays. With excitation at 649–650 nm and emission around 670 nm, Cy5 provides excellent spectral separation from fluorophores such as FITC and Cy3, making it an ideal choice for multiplex fluorescence imaging and advanced immunodetection workflows.

Fluorescence Characteristics

Cy5 delivers bright far-red fluorescence with low background autofluorescence and high sensitivity. It is efficiently excited using both 647 nm krypton/argon lasers and 633 nm helium/neon lasers, providing broad compatibility with confocal microscopes, flow cytometers, and fluorescence imaging systems.

Antibody Formats and Specificity

• Whole IgG antibodies: Purified intact immunoglobulins retain both Fab antigen-binding regions and the Fc domain, providing high affinity and reliable performance across immunodetection applications.
• Cross-adsorbed antibodies: Available pre-adsorbed against serum proteins from multiple species to minimize non-specific binding and cross-reactivity in multiplex experiments.
• High specificity: Validated by immunoelectrophoresis and ELISA for recognition of guinea pig IgG with negligible reactivity toward non-immunoglobulin serum proteins.

Applications and Advantages

• Multiplex fluorescence: Far-red emission enables simultaneous detection with FITC, Cy3, Alexa Fluor® 488, and other fluorophores with minimal spectral overlap.
• Broad application compatibility: Validated for immunofluorescence (IF), immunohistochemistry (IHC-P and IHC-F), immunocytochemistry (ICC), flow cytometry (FCM), and fluorescence microscopy.
• Research proven: Successfully employed in peptide microarray-based epitope mapping and other fluorescence detection platforms requiring high sensitivity and low background.
• Flexible optimization: Typical working dilutions range from 1:20–1:500 depending on sample type, antigen abundance, and imaging conditions.