Triple staining in immunohistochemistry (IHC) allows the detection of 3 different antigens within the same tissue. This technique can be used to visualize these 3 antigens and to analyze their interactions, their locations and their functions. In addition, triple staining saves time compared to 3 simple stainings and also preserves valuable samples using less material, detection can be done on the same tissue section.

The general protocol of IHC triple-staining is based on the polymer method and uses three different enzyme complexes (mostly HRP and alkaline phosphatase) for the three antigens studied in order to obtain independent signals.

IHC triple-staining detection kits for human tissue anti-Mouse and rabbit IgG are optimized for use on paraffin embeded human tissue. These kits permit detection of 2 mouse antibodies and 1 rabbit antibody.

The range of IHC triple-staining kits for human tissues, anti-mouse and rabbit IgG offers enzymatic complexes: Mouse-HRP/Mouse-AP/Rabbit-HRP and several color combinations depending on substrates used: brown, red and green or brown, red and black. You can choose the combination that suits you best.