Lateral flow assay (LFA)

Lateral flow assay (LFA)


Lateral flow tests also known as lateral flow immunochromatographic assays, are simple paper-based devices intended to detect the presence (or absence) of a target analyte in liquid sample (matrix).

Lateral flow assays (LFA) also known as lateral flow immunochromatographic assays, are simple paper-based devices intended to detect the presence (or absence) of a target analyte in liquid sample (matrix) without the need for specialized and costly equipment, though many lab based applications exist that are supported by reading equipment. Typically, these tests are used for medical diagnostics either for home testing, point of care testing, or laboratory use. A widely spread and well known application is the home pregnancy test.
A lateral flow assay (LFA) is composed of four parts: a sample pad, which is the area on which sample is dropped; conjugate pad, on which labeled tags combined with biorecognition elements; reaction membrane containing test line and control line for target antigen-antibody interaction; and absorbent pad, which reserves waste.

In mycology, the most widely used LFA test is the Cryptococcus Antigen Detection Test.

The IMMY CrAg LFA (Cryptococcal Antigen Lateral Flow Assay) is an immunochromatographic dipstick assay for the qualitative and semiquanitative detection of cryptococcal antigen. This lateral flow assay is revolutionizing cryptococcal antigen testing, by delivering analytical sensitivity that is up to 200x more sensitive than other commercial assays. The CrAg LFA is empowering health care providers in all clinical settings with rapid, reliable, and robust diagnostic results.
Unlike other cryptococcal antigen diagnostics on the market, the CrAg LFA has excellent sensitivity across all four serotypes of Cryptococcus, including C. gattii.