CD45 antibody [HI30]
Cat# GTX00526-100ug
Size : 100ug
Marca : Genetex
Contatta il distributore locale :
Telefono : +1 850 650 7790
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | HI30 |
Isotype | IgG1 |
Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Neutralizing/Inhibition |
Reactivity | Human, Baboon, Chimpanzee |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX00526 on CiteAb
PRODUCT
Summary
The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | Assay dependent |
| ICC/IF | Assay dependent |
| IHC-P | Assay dependent |
| IHC-Fr | Assay dependent |
| FACS | Assay dependent |
| IP | Assay dependent |
| Neutralizing/Inhibition | Assay dependent |
Not tested in other applications.
Calculated MW
147 kDa. ( Note )
Product Note
This antibody recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen).
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
Clone Reference
2020; Structural analysis of ischemic stroke thrombi: histological indications for therapy resistance
Application : IHC-P
Reactivity : Human
2019; Divergent Effects of Acute and Prolonged Interleukin 33 Exposure on Mast Cell IgE-Mediated Functions
Application : FACS
Reactivity : Human
2017; Ex vivo-expanded baboon CD39 + regulatory T cells prevent rejection of porcine islet xenografts in NOD-SCID IL-2rγ -/- mice reconstituted with baboon peripheral blood mononuclear cells
Application : IHC-Fr
Reactivity : Baboon
2011; Transgenic expression of human signal regulatory protein alpha in Rag2-/-gamma(c)-/- mice improves engraftment of human hematopoietic cells in humanized mice
Application : FACS
Reactivity : Human
2006; Anti-CD45RO suppresses human immunodeficiency virus type 1 replication in microglia: role of Hck tyrosine kinase and implications for AIDS dementia
Submit a Reference Application : WB, Neutralizing/Inhibition
Reactivity : Human
REVIEW
Clone Reference
2020; Structural analysis of ischemic stroke thrombi: histological indications for therapy resistance
Application : IHC-P
Reactivity : Human
2019; Divergent Effects of Acute and Prolonged Interleukin 33 Exposure on Mast Cell IgE-Mediated Functions
Application : FACS
Reactivity : Human
2017; Ex vivo-expanded baboon CD39 + regulatory T cells prevent rejection of porcine islet xenografts in NOD-SCID IL-2rγ -/- mice reconstituted with baboon peripheral blood mononuclear cells
Application : IHC-Fr
Reactivity : Baboon
2011; Transgenic expression of human signal regulatory protein alpha in Rag2-/-gamma(c)-/- mice improves engraftment of human hematopoietic cells in humanized mice
Application : FACS
Reactivity : Human
2006; Anti-CD45RO suppresses human immunodeficiency virus type 1 replication in microglia: role of Hck tyrosine kinase and implications for AIDS dementia
Application : WB, Neutralizing/Inhibition
Reactivity : Human
2004; Cell surface sialic acids do not affect primary CD22 interactions with CD45 and surface IgM nor the rate of constitutive CD22 endocytosis
Application : IP
Reactivity : Human
2003; Inducible CD40 expression mediates NFkappaB activation and cytokine secretion in human colonic fibroblasts
Application : ICC/IF
Reactivity : Human
2002; CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase
Application : Neutralizing/Inhibition
Reactivity : Human
2001; Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation
Application : WB
Reactivity : Human
2000; Human bronchial intraepithelial T lymphocytes as a distinct T-cell subset: their long-term survival in SCID-Hu chimeras
Application : IHC-Fr
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |