CGRP antibody [4901]

Cat# GTX10987-50ul

Size : 50ul

Marca : Genetex

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Telefono : +1 850 650 7790

Host

Mouse

Clonality

Monoclonal

Clone Name

4901

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr, ELISA, IHC, Neutralizing/Inhibition, RIA

Reactivity

Human, Mouse, Rat, Dog
Package
50 μl
View product citations for antibody GTX10987 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
ELISA 0.25~0.5 μg/ml
IHC 1:100
Neutralizing/Inhibition Assay dependent
RIA Assay dependent
Not tested in other applications.

Positive Control

rat α-CGRP

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

rat α-CGRP peptide. The epitope recognized by the antibody resides within the C-terminal ten amino acids of rat α-CGRP.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcitonin-related polypeptide alpha , CAL6 , CGRP , Cal1 , Calc , RATCAL6 , calcitonin

Cellular Localization

Secreted

Background

Calcitonin gene-related peptide (CGRP), amylin (AMY), and adrenomedullin (ADM) are structurally related peptides of the same family. They are characterized by six to seven amino acid ring structure linked by a disulfide bridge and an amidated C-terminus. CGRP is a 37 amino acid peptide derived from the alternative splicing, which is tissue specific, of the calcitonin gene and may exist in two forms alpha and beta with similar biological functions. Calcitonin gene-related peptide is widely found in both the central and peripheral (sensory and motor neurons) nervous systems. Structural activity studies have found that following peripheral or intracerebroventricular injections of CGRP, several biological actions have been demonstrated that are mediated by the activation of the CGRP receptor subtypes designated CGRP1 and CGRP2. BIBN4096BS, a potent non-peptide antagonist, competitively antagonizes the effects of CGRP in the rat atrium with a potency of about 10-fold higher than that of CGRP (8-37). BIBN4096BS was much less potent at antagonizing the effects of CGRP, proposed to be mediated by the CGRP2 subtype. An orphan receptor originally described as the calcitonin-receptor- like receptor (CRLR) has been identified as a CGRP receptor. Co-expression with a single transmembrane receptor, termed receptor-activity-modifying protein 1 (RAMP1), is required for this receptor to behave as a CGRP1 receptor.

Database

Clone Reference

Li L et al. Brain Behav Immun 2021; 98:265-282 KDM6B epigenetically regulated-interleukin-6 expression in the dorsal root ganglia and spinal dorsal horn contributes to the development and maintenance of neuropathic pain following peripheral nerve injury in male rats.
J Meng et al. J Allergy Clin Immunol 2018; 141 (5):1677-1689.e8 New mechanism underlying IL-31–induced atopic dermatitis
Application : ICC/IF
Reactivity : Mouse
Hashimoto M et al. Front Neural Circuits 2018; 12 (6) Anatomical Evidence for a Direct Projection from Purkinje Cells in the Mouse Cerebellar Vermis to Medial Parabrachial Nucleus
Application : IHC-Fr
Reactivity : Mouse
SL Holtz et al. J Comp Neurol. 2015; 523 (1):139–161 Morphology and Connectivity of Parabrachial and Cortical Inputs to Gustatory Thalamus in Rats
Application : RIA
B Waber-Wenger et al. Histochem Cell Biol 2014; 142 (4):433-47 Sensory innervation of the dorsal longitudinal ligament and the meninges in the lumbar spine of the dog
Reactivity : Dog
Submit a Reference

REVIEW

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Clone Reference
Li L et al. Brain Behav Immun 2021; 98:265-282 KDM6B epigenetically regulated-interleukin-6 expression in the dorsal root ganglia and spinal dorsal horn contributes to the development and maintenance of neuropathic pain following peripheral nerve injury in male rats.
J Meng et al. J Allergy Clin Immunol 2018; 141 (5):1677-1689.e8 New mechanism underlying IL-31–induced atopic dermatitis
Application : ICC/IF
Reactivity : Mouse
Hashimoto M et al. Front Neural Circuits 2018; 12 (6) Anatomical Evidence for a Direct Projection from Purkinje Cells in the Mouse Cerebellar Vermis to Medial Parabrachial Nucleus
Application : IHC-Fr
Reactivity : Mouse
SL Holtz et al. J Comp Neurol. 2015; 523 (1):139–161 Morphology and Connectivity of Parabrachial and Cortical Inputs to Gustatory Thalamus in Rats
Application : RIA
B Waber-Wenger et al. Histochem Cell Biol 2014; 142 (4):433-47 Sensory innervation of the dorsal longitudinal ligament and the meninges in the lumbar spine of the dog
Reactivity : Dog
KE Bowler et al. Neuroscience 2013; 228:271-82 Evidence for anti-inflammatory and putative analgesic effects of a monoclonal antibody to calcitonin gene-related peptide
Application : Neutralizing/Inhibition
Fang Z et al. PLoS One 2013; 8 (8):e72738 Effect of CGRP-adenoviral vector transduction on the osteoblastic differentiation of rat adipose-derived stem cells.
Application : WB
Reactivity : Human
J Zeller et al. Br J Pharmacol 2008; 155 (7):1093-103 CGRP function-blocking antibodies inhibit neurogenic vasodilatation without affecting heart rate or arterial blood pressure in the rat
Application : ELISA
HC Wong et al. Hybridoma 1993; 12 (1):93-106 Monoclonal Antibody to Rat a-CGRP: Production, Characterization, and In Vivo Immunoneutralization Activity
Application : IHC
Reactivity : Dog
SDS
PBS.pdf
Sodium Azide.pdf