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> ATG3 Antibody

ATG3 Antibody

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Cat# ASM10537-A655-100

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Marca : Abcepta

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  • ICC/IF - ATG3 Antibody ASM10537
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody (ASM10537). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody (ASM10537) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.
  • WB - ATG3 Antibody ASM10537
    Western blot analysis of Human HeLa cell lysates showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody (ASM10537). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody (ASM10537) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9NT62
Other Accession NP_001265641.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG3 Polyclonal
Target/Specificity Detects ~35 kDa.
Other Names ATG3 Antibody, ATG3 autophagy related 3 homolog (S. cerevisiae) Antibody, Ubiquitin-like-conjugating enzyme ATG3 Antibody, APG3-like Antibody, APG3L Antibody, APG3, S. cerevisiae, homolog of Antibody, APG3 like Antibody, Apg 3 Antibody, Autophagy-related protein 3 Antibody, Apg3p Antibody, APG3 Antibody, ATG 3 Antibody, hApg3 Antibody, Autophagy related protein 3 Antibody, Autophagy 3, S. cerevisiae, homolog of Antibody, autophagy related 3 Antibody, ATG3_HUMAN Antibody, Protein PC3-96 Antibody, Autophagy Apg3p/Aut1p like Antibody, PC3 96 Antibody, APG3 autophagy 3 like Antibody, ATG3 autophagy related 3 homolog Antibody
Immunogen Synthetic peptide from the mid-protein of Human ATG3
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-671 was sufficient for detection of ATG3 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm