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> ATG4A Antibody

ATG4A Antibody

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Cat# ASM10510-A655-100

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Marca : Abcepta

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  • ICC/IF - ATG4A Antibody ASM10510
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
  • WB - ATG4A Antibody ASM10510
    Western blot analysis of Human HeLa cell lysates showing detection of 45.3 kda ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (ASM10510) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kda.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q8WYN0
Other Accession NP_001308216.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG4A Polyclonal
Target/Specificity Detects ~48 kDa.
Other Names ATG4 autophagy related 4 homolog A (S. cerevisiae) Antibody, MGC107179 Antibody, AV169859 Antibody, Autophagy-related cysteine endopeptidase 2 Antibody, hAPG4A Antibody, EC:3.4.22.- Antibody, ATG4A Antibody, Autophagin 2 Antibody, Autophagy related protein 4 homolog A Antibody, Cysteine protease ATG4A Antibody, AUTL2 Antibody, Autl2 Antibody, Autophagy-related protein 4 homolog A Antibody, Atg4al Antibody, Autophagin-2 Antibody, Autophagy related 4A cysteine peptidase Antibody, AUT like 2 cysteine endopeptidase Antibody, Autophagy related cysteine endopeptidase 2 Antibody, Apg4a Antibody, AUT-like 2 cysteine endopeptidase Antibody, APG4A Antibody, AI627006 Antibody, ATG4A_HUMAN Antibody,
Immunogen Synthetic peptide from the N-terminal of Human ATG4A (aa. 58-70)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-629 was sufficient for detection of ATG4A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm