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> ATG4C Antibody

ATG4C Antibody

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Cat# ASM10514-A655-100

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Marca : Abcepta

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  • ICC/IF - ATG4C Antibody ASM10514
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4C Antibody. (D) Composite.
  • WB - ATG4C Antibody ASM10514
    Western blot analysis of Human HEK293T cell lysates showing detection of 53 kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HEK293T cell lysates. Load: 15 µg . Block: 2% GE Ashermsham in 1X TBST. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 minutes in RT. Predicted/Observed Size: 53 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q96DT6
Other Accession NP_116241.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG4C Polyclonal
Target/Specificity Detects ~50 kDa.
Other Names AUT like 1cysteine endopeptidase (S. cerevisiae) Antibody, APG4 autophagy 4 homolog C Antibody, Cysteine protease ATG4C Antibody, APG4-C Antibody, Autophagy-related cysteine endopeptidase 3 Antibody, EC:3.4.22.- Antibody, ATG 4C Antibody, AUT like 1, cysteine endopeptidase Antibody, EC 3.4.22 Antibody, APG4C Antibody, Autophagin-3 Antibody, OTTHUMP00000010715 Antibody, AUTL3 Antibody, APG4 autophagy 4 homolog C (S. cerevisiae) Antibody, Autophagy related cysteine endopeptidase 3 Antibody, ATG4C Antibody, Autophagin 3 Antibody, ATG4 autophagy related 4 homolog C Antibody, AUT like 3 cysteine endopeptidase Antibody, AUTL1 Antibody, ATG4C_HUMAN Antibody, AUT (S. cerevisiae) like 1, cysteine endopeptidase Antibody, Autophagy-related protein 4 homolog C Antibody, Autophagy related protein 4 homolog C Antibody, ATG4 autophagy related 4 homolog C (S. cerevisiae) Antibody, Autophagy related 4C cysteine peptidase Antibody, Atg4c Antibody, APG4 C Antibody, FLJ14867 Antibody, AUT-like 3 cysteine endopeptidase Antibody,
Immunogen Synthetic peptide from the C-terminal of Human ATG4C (aa. 296-306)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-633 was sufficient for detection of ATG4C in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm