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> Beclin 2 Antibody

Beclin 2 Antibody

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Cat# ASM10491-A655-100

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  • ICC/IF - Beclin 2 Antibody ASM10491
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10491). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10491) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.
  • WB - Beclin 2 Antibody ASM10491
    Western blot analysis of Human 293Trap cell lysates showing detection of ~48.1 kDa Beclin2 protein using Rabbit Anti-Beclin2 Polyclonal Antibody (ASM10491). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin2 Polyclonal Antibody (ASM10491) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.
  • WB - Beclin 2 Antibody ASM10491
    Western blot analysis of Rat Liver showing detection of ~48kDa Beclin-2 protein using Rabbit Anti-Beclin-2 Polyclonal Antibody (ASM10491). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin-2 Polyclonal Antibody (ASM10491) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~48kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession A8MW95
Other Accession NP_001277622.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human Beclin 2 Polyclonal
Target/Specificity Predicted molecular weight at ~48.1kDa. Observed bands in the 50-55kDa range, varied by lysates.
Other Names BECN2 Antibody, Beclin-2 Antibody, Beclin-1 like protein 1 Antibody
Immunogen Synthetic peptide from the mid-protein of human Beclin 2
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-602 was sufficient for detection of Beclin2 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm