Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Adenosine receptor A1 Antibody

Adenosine receptor A1 Antibody

Stampa Stampa
Entra

Cat# ASM10557-A680-100

Size : Onrequest

Marca : Abcepta

Contatta il distributore locale :


Telefono : +1 850 650 7790

 
  • ICC/IF - Adenosine receptor A1 Antibody ASM10557
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (ASM10557). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (ASM10557) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Adenosine receptor A1 Antibody (D) Composite.
  • WB - Adenosine receptor A1 Antibody ASM10557
    Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~36.5 kDa Adenosine receptor A1 protein using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (ASM10557). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (ASM10557) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36.5 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P30542
Other Accession NP_000665.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human Adenosine receptor A1 Polyclonal
Target/Specificity Detects ~37 kDa.
Other Names ADORA1 Antibody, Adenosine receptor A1 Antibody, Adenosine A1 Receptor Antibody, A1AR Antibody, A1R Antibody, AA1R Antibody, ADORA 1 antibody, RDC 7 Antibody, RDC7 Antibody, Ri Antibody, Adenosine receptor (A1) Antibody
Immunogen Synthetic peptide from the C-terminal of Human Adenosine receptor A1
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-702 was sufficient for detection of Adenosine receptor A1 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Multi-Pass Membrane Protein