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> CD74 Antibody

CD74 Antibody

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Cat# ASM10175-A680-100

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Marca : Abcepta

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  • ICC/IF - CD74 Antibody ASM10175
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8 (ASM10175). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (ASM10175) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.
  • WB - CD74 Antibody ASM10175
    Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8 (ASM10175). Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (ASM10175) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P04233
Other Accession NP_001020329.1
Host Mouse
Isotype IgG2a
Reactivity Human
Clonality Monoclonal
Description Mouse Anti-Human CD74 Monoclonal IgG2a
Target/Specificity Detects ~33 kDa protein.
Other Names DHLAG Antibody, HLA DR gamma Antibody, HLADG Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody, CD 74 Antibody, CD74 Antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Antibody, CD74 antigen Antibody, CD74 molecule Antibody, CD74 molecule, major histocompatibility complex, class II invariant chain Antibody, CLIP Antibody, DHLAG Antibody, Gamma chain of class II antigens Antibody, HG2A_HUMAN Antibody, HLA class II histocompatibility antigen gamma chain Antibody, HLA DR antigens associated invariant chain Antibody, HLA DR gamma Antibody, HLA-DR antigens-associated invariant chain Antibody, HLA-DR-gamma Antibody, HLADG Antibody, HLADR antigens associated invariant chain Antibody Ia antigen associated invariant chain Antibody, Ia antigen-associated invariant chain Antibody, Ia associated invariant chain Antibody, Ia gamma Antibody, Ii Antibody, Invariant polypeptide of major histocompatibility complex class II antigen associated Antibody, la-gamma Antibody, Major histocompatibility complex class II invariant chain Antibody, MHC HLA DR gamma chain Antibody, MHC HLA-DR gamma chain Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody
Clone Names 1B8
Immunogen Human CD74 invariant chain synthetic peptide.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SMC-267 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Cellular Localization Cell Membrane | Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Golgi Apparatus | Endosome | Lysosome