GLUT2 Antibody

Cat# ASM10554-A680-100

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Marca : Abcepta

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  • ICC/IF - GLUT2 Antibody ASM10554
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GLUT2 Polyclonal Antibody (ASM10554). Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody (ASM10554) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GLUT2 Antibody (D) Composite.
  • WB - GLUT2 Antibody ASM10554
    Western blot analysis of Rat Liver showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody (ASM10554). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody (ASM10554) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~37kDa.
  • WB - GLUT2 Antibody ASM10554
    Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody (ASM10554). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody (ASM10554) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~72kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P11168
Other Accession NP_000331.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human GLUT2 Polyclonal
Target/Specificity Predicted molecular weight at ~57.5kDa. Observed molecular weight at 60-70kDa and 38-45kDa.
Other Names Glucose Transporter 2 Antibody, Glucose Transporter GLUT2 Antibody, BLUT-2 Antibody, GTR_Human Antibody, SLC2a2 Antibody, Solute Carrier Family 2 (facilitated glucose transporter) member 2 Antibody
Immunogen Synthetic peptide from the C-terminal of human GLUT2
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-697 was sufficient for detection of GLUT2 on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Membrane