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> IRGM Antibody

IRGM Antibody

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Cat# ASM10541-A633-100

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  • ICC/IF - IRGM Antibody ASM10541
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody (ASM10541). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (ASM10541) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.
  • WB - IRGM Antibody ASM10541
    Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody (ASM10541). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (ASM10541) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession A1A4Y4
Other Accession NP_001139277.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human IRGM Polyclonal
Target/Specificity Detects ~20 kDa.
Other Names Immunity related GTPase family M Antibody Iipg3 Antibody Interferon inducible protein 1 Antibody, LRG-47-like protein Antibody, Immunity-related GTPase family M protein 1 Antibody, IFI1 Antibody, Irgm Antibody, IRGM_HUMAN Antibody, LRG47 Antibody, Interferon-inducible protein 1 Antibody, IRGM1 Antibody, LRG 47 Antibody, IRGM Antibody, Iigp3 Antibody, Immunity-related GTPase family M protein Antibody, LRG-47 Antibody, MGC149263 Antibody, Immunity related GTPase family M protein 1 Antibody, LPS-stimulated RAW 264.7 macrophage protein 47 homolog Antibody
Immunogen Synthetic peptide from the N-terminal of Human IRGM
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-681 was sufficient for detection of IRGM in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Golgi Apparatus Membrane | Cell Membrane | Cytoplasmic Vesicle | Phagosome Membrane | Cytoplasmic Vesicle | Autophagosome Membrane | Cell Projection | Phagocytic Cup