ASM10298-A633-100 | Laforin Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Laforin Monoclonal Antibody, Clone S84-37 (ASM10298). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Laforin Monoclonal Antibody (ASM10298) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Laforin Antibody (D) Composite.
Western Blot analysis of Human Hippocampus showing detection of ~40 kDa and 45 kDa Laforin protein using Mouse Anti-Laforin Monoclonal Antibody, Clone S84-37 (ASM10298). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Human Hippocampus. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Laforin Monoclonal Antibody (ASM10298) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~40 kDa and 45 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	O95278
Other Accession	NP_001018051.1
Host	Mouse
Isotype	IgG1
Reactivity	Human
Clonality	Monoclonal
Description	Mouse Anti-Human Laforin Monoclonal IgG1
Target/Specificity	Detects ~40kDa.
Other Names	EPM2 Antibody, Epilepsy progressive myoclonus type 2 Lafora disease (laforin) Antibody, Epilepsy progressive myoclonus type 2A Lafora disease (laforin) Antibody, EPM2 Antibody, Epm2a Antibody, Lafora PTPase Antibody, LAFPTPase Antibody, LD Antibody, LDE Antibody, MELF Antibody
Clone Names	S84-37
Immunogen	Fusion protein amino acids 1-331 (full-length) of human Laforin. Rat: 90% identity (296/327 amino acids identical). Mouse: 89% identity (295/327 amino acids identical)
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-466 was sufficient for detection of Laforin in 20 µg of human brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Endoplasmic Reticulum

Laforin Antibody