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> VDCA-1 Antibody

VDCA-1 Antibody

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Cat# ASM10552-A633-100

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  • ICC/IF - VDCA-1 Antibody ASM10552
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Mitochondrion Outer Membrane, Cell Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VDCA-1 Antibody. (D) Composite.
  • WB - VDCA-1 Antibody ASM10552
    Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~30.7 kDa VDCA-1 protein using Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VDCA-1 Polyclonal Antibody (ASM10552) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30.7 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P21796
Other Accession NP_003365.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human VDCA-1 Polyclonal
Target/Specificity Detects ~37 kDa.
Other Names N2441 Antibody, POR1 Antibody, Vdac1 Antibody, VDAC1 Antibody, MGC111064 Antibody, Outer mitochondrial membrane protein porin 1 Antibody, OMP2 Antibody, YNL055C Antibody, VDAC1_HUMAN Antibody, YNL2441C Antibody, VDAC-1 Antibody, Voltage dependent anion channel 1 Antibody, Voltage-dependent anion-selective channel protein 1 Antibody, Voltage dependent anion selective channel protein 1 Antibody, Plasmalemmal porin Antibody, Porin 31HL Antibody, Mitochondrial Porin Antibody, Porin 31HM Antibody, hVDAC1 Antibody, VDAC Antibody, VDAC1/porin Antibody, VDAC1 / Porin Antibody
Immunogen Synthetic peptide from the N-terminal of Human VDCA-1
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-695 was sufficient for detection of VDCA-1 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion Outer Membrane | Multi-Pass Membrane Protein | Cell Membrane | Multi-Pass Membrane Protein | Membrane Raft | Multi-Pass Membrane Protein