ATG5 Antibody

Cat# ASM10501-A700-100

Size : Onrequest

Marca : Abcepta

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Telefono : +1 850 650 7790

  • ICC/IF - ATG5 Antibody  ASM10501
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody (ASM10501). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (ASM10501) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite.
  • WB - ATG5 Antibody  ASM10501
    Western blot analysis of Human HeLa cell lysates showing detection of ~32.4kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody (ASM10501). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (ASM10501) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~32.4kDa. Other Band(s): ~105kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9H1Y0
Other Accession NP_001273035.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG5 Polyclonal
Target/Specificity Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
Other Names APG5-like Antibody, ASP Antibody, hAPG5 Antibody
Immunogen Synthetic peptide from the N-terminal of human ATG5
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-612 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm