ASM10547-A700-100 | beta Actin Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beta Actin Polyclonal Antibody (ASM10547). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beta Actin Polyclonal Antibody (ASM10547) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beta Actin Antibody (D) Composite.
Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~41.7kDa beta Actin protein using Rabbit Anti-beta Actin Polyclonal Antibody (ASM10547). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-beta Actin Polyclonal Antibody (ASM10547) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~41.7kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	P60709
Other Accession	NP_001092.1
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human beta Actin Polyclonal
Target/Specificity	Predicted molecular weight at ~41.7kDa. A double band may be observed.
Other Names	A X actin like protein Antibody, ACTB Antibody, ACTB_HUMAN Antibody, Actin Antibody, Actin cytoplasmic 1 Antibody, actin beta Antibody, Actx Antibody, Beta-actin Antibody, PS1TP5 binding protein 1 Antibody
Immunogen	Synthetic peptide from the mid-protein range of human beta Actin
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-687 was sufficient for detection of beta Actin on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Cytoskeleton

beta Actin Antibody