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> ESR1 Antibody

ESR1 Antibody

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Cat# ASM10562-A700-100

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  • ICC/IF - ESR1 Antibody ASM10562
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ESR1 Polyclonal Antibody (ASM10562). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ESR1 Polyclonal Antibody (ASM10562) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ESR1 Antibody. (D) Composite.
  • WB - ESR1 Antibody ASM10562
    Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~66.2 kDa ESR1 protein using Rabbit Anti-ESR1 Polyclonal Antibody (ASM10562). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ESR1 Polyclonal Antibody (ASM10562) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~66.2 kDa. Other Band(s): 50 kDa is isoform 2.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P03372
Other Accession NP_000116.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ESR1 Polyclonal
Target/Specificity Detects ~66 kDa.
Other Names Estrogen receptor alpha Antibody, ER alpha Antibody, ER-alpha Antibody, Estrogen receptor alpha delta 3*, 4, 5, 6, 7*/819 2 isoform Antibody, NR3A1 Antibody, Nuclear receptor subfamily 3 group A member 1 Antibody, Estrogen receptor alpha delta 4*, 5, 6, 7*/654 isoform Antibody, ESR1_HUMAN Antibody, ESTRR Antibody, Era Antibody, Estrogen receptor alpha delta 3*, 4, 5, 6, 7*, 8*/941 isoform Antibody, Estrogen receptor 1 Antibody, Estrogen receptor alpha 3*, 4, 5, 6, 7*/822 isoform Antibody, DKFZp686N23123 Antibody, Estrogen receptor alpha delta 4*, 5, 6, 7, 8*/901 isoform Antibody, Estrogen receptor Antibody, Estrogen receptor alpha E1 E2 1 2 Antibody, ESR1 Antibody, Estrogen receptor alpha E1 N2 E2 1 2 Antibody, Estrogen nuclear receptor alpha Antibody, ESR Antibody, ER Antibody, ESRA Antibody, Estrogen receptor alpha delta 4 +49 isoform Antibody, Estradiol receptor Antibody
Immunogen Synthetic peptide from the mid-protein of Human ESR1
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-708 was sufficient for detection of ESR1 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus | Cytoplasm | Cell Membrane | Peripheral Membrane Protein | Cytoplasmic Side