ASM10506-A700-100 | GABARAPL1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL1 Polyclonal Antibody (ASM10506). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (ASM10506) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL1 Antibody (D) Composite.
Western blot analysis of Human HeLa cell lysates showing detection of ~14kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (ASM10506). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (ASM10506) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~14kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	Q9H0R8
Other Accession	NP_113600.1
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human GABARAPL1 Polyclonal
Target/Specificity	Detects ~14-16kDa. Other observed bands at ~32kDa.
Other Names	APG8 like Antibdoy, ATG8 Antibody, GABA(A) Receptor Associated Protein Like 1 Antibody, Glandular epithelial cell protein 1 Antibody
Immunogen	Synthetic peptide from the C-terminal of human GABARAPL1
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-623 was sufficient for detection of GABARAPL1 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Cytoskeleton \| Endoplasmic Reticulum \| Golgi Apparatus \| Cytoplasmic Vesicle \| Autophagosome

GABARAPL1 Antibody