Malin Antibody

Cat# ASM10278-A700-100

Size : Onrequest

Marca : Abcepta

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Telefono : +1 850 650 7790

  • ICC/IF - Malin Antibody ASM10278
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 (ASM10278). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody (ASM10278) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite.
  • WB - Malin Antibody ASM10278
    Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 (ASM10278). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody (ASM10278) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q6VVB1
Other Accession NP_940988.2
Host Mouse
Isotype IgG1
Reactivity Human
Clonality Monoclonal
Description Mouse Anti-Human Malin Monoclonal IgG1
Target/Specificity Detects ~42kDa.
Other Names E3 ubiquitin-protein ligase NHLRC1 Antibody, NHLRC 1 Antibody, NHL repeat containing 1 Antibody, EPM2A Antibody, EPM2B Antibody, MGC119262 Antibody, MGC119264 Antibody, MGC119265 Antibody, NHL repeat containing protein 1 Antibody
Clone Names S85-18
Immunogen Fusion protein amino acids 2-125 (N-terminus encompassing RING domain) of human Malin. 86% identical to rat, and 77% identical to mouse.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-444 was sufficient for detection of malin in 20 µg of transiently (malin) transfected COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Nucleus