ASM10118-A700-100 | Synaptophysin (native) Antibody Clinisciences

Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (ASM10118). Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x.
Western Blot analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11 (ASM10118). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa.
Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (ASM10118). Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x.
Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (ASM10118). Tissue: Fetal brain tissue. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC
Primary Accession	P08247
Other Accession	NP_003170.1
Host	Mouse
Isotype	IgG1
Reactivity	Human
Clonality	Monoclonal
Description	Mouse Anti-Human Synaptophysin (native) Monoclonal IgG1
Target/Specificity	Detects ~38kDa.
Other Names	SYPH Antibody, Sypl Antibody, Syn p38 Antibody, Synaptophysin Antibody, Major synaptic vesicle protein p38 Antibody
Clone Names	EP10
Immunogen	Immunoprecipitate of human brain
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-178 was sufficient for detection of Synaptophysin in 10 µg of human brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasmic Vesicle \| Secretory Vesicle \| Synaptic Vesicle Membrane \| Cell Junction \| Synapse

Synaptophysin (native) Antibody

Cat# ASM10118-A700-100

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Marca : Abcepta

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