Rb (RB1) (612-928) Mouse Monoclonal Antibody [Clone ID: 3H9]
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Specifications
Product Data | |
Clone Name | 3H9 |
Applications | IHC, IP, WB |
Recommended Dilution | Western blot: 5-10 μg/ml. Immunohistochemistry on paraffin sections: 5 μg/ml; Heat treatment is necessary. Microwave oven; 2 times for 10 minutes each in citrate buffer (pH 6.5). Immunoprecipitation: 1-5 μg / 200-300 μl of cell extract. For details see protocols below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant human Rb protein corresponding to amino acids 612-928 |
Specificity | This antibody reacts with retinoblastoma gene product (110-115 kDa). |
Formulation | PBS containing 50% glycerol, pH 7.2 State: Azide Free State: Liquid Ig fraction without preservatives |
Concentration | lot specific |
Purification | Protein A Sepharose |
Conjugation | Unconjugated |
Storage | Store at 2 - 8 °C. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | Mutation of the retinoblastoma tumor suppressor gene alone is sufficient to cause retinoblastoma in humans, suggesting that it might play a role in the normal coordination of cell proliferation and cell death. Deletion or mutational inactivation of the retinoblastoma tumor suppressor protein (Rb) is correlated with the genesis of a variety of human cancers including retinoblastoma, osteosarcoma, and carcinomas of the breast, bladder, and lung. Rb protein is phosphorylated by cyclinD-Cdk4/Cdk6 and cyclinA/cyclinE-Cdk2 during the G1/S transition. This phosphorylation causes the inactivation of the growth inhibitory functions of Rb. Rb undergoes phosphorylation and functional inactivation, permitting the cell to proceed into late G1. |
Synonyms | Retinoblastoma 1, Rb, p105-Rb, pRb, pp110, OSRC |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation Immunohistochemical staining for paraffin-embedded sections: SAB method |
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