ASM10356-A680-100 | HSP70 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody (ASM10356). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody (ASM10356) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356) at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.
Western blot analysis of Human, Rat brain cell lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356). Load: 2 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356) at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
Western blot analysis of Mouse Pam212 cells showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody (ASM10356). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody (ASM10356) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (ASM10356) at 1:1000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, IP, ICC/IF
Primary Accession	P08107
Other Accession	NP_005336.3
Host	Rabbit
Reactivity	Plant, Beluga, Shark, Carp, Human, Mouse, Rat, Hamster, Monkey, Pig, Bovine, Dog, Fish, Sheep, Guinea Pig
Clonality	Polyclonal
Description	Rabbit Anti-Human HSP70 Polyclonal
Target/Specificity	Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.
Other Names	HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Immunogen	Full length protein HSP70
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-103 was sufficient for detection of HSP70 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization	Cytoplasm

HSP70 Antibody

Cat# ASM10356-A680-100

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