Anti-Human RANTES
Cat# R130-25
Size : 25ug
Marca : Leinco Technologies
AntiHuman RANTES
AntiHuman RANTES
Product No.: R130
Target RANTES Product Type Polyclonal Antibody Alternate Names CCL5, D17S136E, MGC17164, SCYA5, SISDelta, TCP228 Applications ICC , IHC FFPE , N , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human RANTES (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831706 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human RANTES this polyclonal antibody can be used at a concentration of 0.1 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human RANTES is 1 ng/lane under either reducing or nonreducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 515 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human RANTES bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human RANTES (at 0.01 ng/ml) is 0.1 0.4 µg/ml. Immunocytochemistry: Suitable for use at concentration of 515 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat AntiHuman Regulated upon Activation Normal T Cell Express Sequence (RANTES) recognizes Human RANTES. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background RANTES also known as Chemokine (CC motif) ligand 5 (CCL5), a CCchemokine1 is an important mediators of the immune and inflammatory response.2 It is also a potent chemoattractant for T cells 3 and eosinophils.4 RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayedtype hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.5 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Lee, SC. et al. (2004) J Neurochem. 90: 297 2. Borkowski, J. et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201 3. Geiger, H. et al. (2005) Kidney Blood Press Res 28: 48 4. Hirata, I. et al.(2009) Dig Dis Sci. 54(6):124752. 5. Emilie, D. et al. (1994) J Exp Med. 179: 1689 |