Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Non-infected (–) and infected (+) C6/36 whole cell extract (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

The data was published in the journal Virulence in 2018. PMID: 29338543

The data was published in the journal Virulence in 2018. PMID: 29338543

The data was published in the journal Cell Rep in 2019.PMID: 31067454