Di-4-ANEPPS is a fast-responding membrane potential dye (1). Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye. Di-4-ANEPPS is internalized in cells rapidly, so it is mainly used for short-term experiments. We also offer Di-8-ANEPPS (catalog number 61012), which is more lipophilic and better retained in the outer leaflet of the plasma membrane.

• λ_Ex/λ_Em (MeOH) = 496/705 nm*
• Orange red solid soluble in DMF, ethanol or DMSO
• Store at 4°C and protect from light, especially in solution
• C₂₈H₃₆N₂O₃S
• MW: 481
• [90134-00-2]

* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.