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> GAD1 / GAD67 (GABAergic Neuronal Marker) (GAD1/2563), 0.2mg/mL

GAD1 / GAD67 (GABAergic Neuronal Marker) (GAD1/2563), 0.2mg/mL

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Cat# BNUB2563-100

Size : 100uL

Marca : Biotium

GAD1 / GAD67 (GABAergic Neuronal Marker) (GAD1/2563), 0.2mg/mL

GAD1 / GAD67 Monoclonal Mouse Antibody (GAD1/2563)

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Formalin-fixed paraffin-embedded human Brain stained with GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563).Formalin-fixed paraffin-embedded human Brain stained with GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563).
SDS Page Analysis Purified GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563).Confirmation of Purity and Integrity of Antibody.SDS Page Analysis Purified GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563).Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.Analysis of Protein Array containing more than 19000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.
  1. Formalin-fixed paraffin-embedded human Brain stained with GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563).
  2. SDS Page Analysis Purified GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563).Confirmation of Purity and Integrity of Antibody.
  3. Analysis of Protein Array containing more than 19000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2563) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.
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Product Description

This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2563, Anti-GAD1|GAD67 (GAD1/2563) 

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC74CF®740742/767633-685775/50CF®740 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free		N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
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Product Attributes

Antibody number
#2563
Size
50 uL, 100 uL, 250 uL, 500 uL
Antibody reactivity (target)
GAD1, GAD67
Antibody type
Primary
Host species
Mouse
Clonality
Monoclonal
Clone
GAD1/2563
Isotype
IgG2b, kappa
Molecular weight
67 kDa
Synonyms
67kDa glutamic acid decarboxylase; CPSQ1; DCE1; GAD67; GAD1; Glutamate decarboxylase 1; SCP
Human gene symbol
GAD1
Entrez gene ID
2571
SwissProt
Q99259
Unigene
420036
Immunogen
Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)
Verified antibody applications
Flow (intracellular) (verified)
Antibody target cellular localization
Cytoplasmic, Plasma membrane, Vesicular
Species reactivity
Human
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, ELISA: For coating order antibody without BSA, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Western: 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user
Positive control
K-562 or HEK293 Cells. Pancreas
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Validated in protein array
Monospecific
Antibody research areas
Neuroscience
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression
Brain

Documents, Protocols, SDS and COA

Datasheet
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COA
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