Membrane-based antibody array for detection 43 apoptosis biomarkers, including Caspase 3, Caspase 8, bad, bax, bcl-2, etc.
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Human Apoptosis Array C1
Human Apoptosis Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Apoptosis, IGF Signaling, PI3K-AKT Signaling, p53 Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| bad
| bax
| bcl-2
| bcl-w
| BID
|
| BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
| cIAP-2
| Cytochrome C
| DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
| HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
| IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
| IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
| p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
| TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (podocyte lysates)
- Year:
- 2024
- Journal Name:
- International Journal of Biological Sciences
- PubMed ID:
- 38250156
5,6,7,4'-Tetramethoxyflavone, a Dietary Polymethoxyflavone, Exerts Antitumor Effect on HeLa Xenografts in Mice
- Species:
- Human
- Sample Type:
- Other / Unspecified (HeLa tumor samples xenografts)
- Year:
- 2025
- Journal Name:
- Food Science & Nutrition
- PubMed ID:
- 41179362
Polyphenolic Carbon Quantum Dots with Intrinsic Reactive Oxygen Species Amplification for Two-Photon Bioimaging and In Vivo Tumor Therapy
- Species:
- Mouse
- Sample Type:
- B16F10 cell
- Year:
- 2023
- Journal Name:
- Acs Applied Materials & Interfaces
In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cells (HUVECs)
- Year:
- 2023
- Journal Name:
- Drug Design Development and Therapy
Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4'-tetramethoxyflavone against HeLa cancer cells
- Species:
- Human
- Sample Type:
- Other / Unspecified (total protein extracted from HeLa cells)
- Year:
- 2024
- Journal Name:
- Heliyon
- PubMed ID:
- 39449708
Fraisinib: a calixpyrrole derivative reducing A549 cell-derived NSCLC tumor in vivo acts as a ligand of the glycine-tRNA synthase, a new molecular target in oncology
- Species:
- Human
- Sample Type:
- Other / Unspecified (A549 cell protein extracts)
- Year:
- 2023
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 38235113
The apoptotic effects of Brucea javanica fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation
- Species:
- Human
- Sample Type:
- HT29 cell
- Year:
- 2022
- Journal Name:
- Drug Design Development and Therapy
α-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-κB and Hsp70 protein modulation in vitro, and tumor reduction in vivo
- Species:
- Human
- Sample Type:
- MCF-7 cell
- Year:
- 2022
- Journal Name:
- Drug Design Development and Therapy
Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line
- Species:
- Human
- Sample Type:
- SKOV-3 cell
- Year:
- 2020
- Journal Name:
- International Journal of Nanomedicine
- PubMed ID:
- 33235450
Specific Forms of Graphene Quantum Dots Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells
- Species:
- Human
- Sample Type:
- MCF-7 cell, BT-474 cell，T-47D cell，MDA-MB-231 cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences