Human CD19 Antibody : LE/AF
Cat# OASB00929
Size : 0.5mg
Marca : Aviva Systems Biology
Human CD19 Antibody : LE/AF (OASB00929)
| Datasheets/Manuals | Printable datasheet for Human CD19 Antibody : LE/AF (OASB00929) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | SJ25-C1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IP, BL |
| Additional Information | Description: CD19 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed at all stages of B cell differentiation except terminally differentiated plasma cells. It is also present on the cell surface of follicular dendritic cells. CD19 associates with CD21, CD81, Leu 13 and/or MHC Class II molecules to form a signal transduction complex on the B cell surface. This signaling complex modulates the activation threshold for the B cell antigen receptor (BCR). |
| Reconstitution and Storage | Store at -20C |
| Immunogen | NALM-1 and NALM-16 leukemia cell line |
| Concentration | 0.5 mg/mL |
| Specificity | CD19 |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate R-phycoerythrin conjugate-Texas Red® Allophycocyanin conjugate Spectral Red conjugate Cyanine 5 conjugate R-phycoerythrin-Cyanine 5.5 conjugate R-phycoerythrin-Cyanine 7 conjugate Allophycocyanin -Cyanine 5.5 conjugate Allophycocyanin -Cyanine 7 conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation |
| Other Applications Data | Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 100 tests in 1.0 mL ofPBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and Allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine5.5 (R- PE- CY 5.5), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), Allophycocyanin - Cyanine 5.5 (APCCY 5.5) and Allophycocyanin - Cyanine 7 (APC- CY 7)conjugates are supplied as 100 tests in 1.0 mLof PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Gene Symbol | CD19 |
|---|---|
| Gene Full Name | B-lymphocyte antigen CD19 |
| Alias Symbols | B4, CVID3 |
| NCBI Gene Id | 930 |
| Protein Name | B-lymphocyte antigen CD19 |
| Description of Target | Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Uniprot ID | P15391 |
| Protein Accession # | NP_001171569.1 |
| Nucleotide Accession # | NM_001178098.1 |
