RayBio® G-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
Human Growth Factor Array G1
Human Growth Factor Array G1
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 41 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| bFGF
| beta-NGF
| EGF
| EGFR
|
FGF-4
| FGF-6
| FGF-7 (KGF)
| GCSF
| GDNF
|
GM-CSF
| HB-EGF
| HGF
| IGFBP-1
| IGFBP-2
|
IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
| IGF-1 R
|
IGF-2
| M-CSF
| M-CSF R
| NT-3
| NT-4
|
PDGF R alpha
| PDGF R beta
| PDGF-AA
| PDGF-AB
| PDGF-BB
|
PLGF
| SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
|
TGF beta 2
| TGF beta 3
| VEGF-A
| VEGFR2
| VEGFR3
|
VEGF-D
| ||||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Growth Factor Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-GF-G1-4.gal)
GAL (GAL-AAH-GF-G1-8.gal)
Excel (AAH-GF-G1_Analysis_Tool.xls)
citations
Tissue-engineered tendon nano-constructs for repair of chronic rotator cuff tears in large animal models
- Species:
- Human
- Sample Type:
- human mesenchymal stem cells (hMSCs)
- Year:
- 2022
- Journal Name:
- Bioengineering & translational medicine
Human amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor
- Species:
- Human
- Sample Type:
- Conditioned media (from hAMSCs)
- Year:
- 2018
- Journal Name:
- Stem Cell Research and Therapy
Renal Extracellular Matrix Scaffolds From Discarded Kidneys Maintain Glomerular Morphometry and Vascular Resilience and Retains Critical Growth Factors
- Species:
- Human
- Sample Type:
- Tissue Lysate (Kidney and renal kidney extracellular decellularized kidney scaffolds)
- Year:
- 2015
- Journal Name:
- Transplantation
- PubMed ID:
- 26018349
The Differentiation of Human Adipose-Derived Stem Cells towards a Urothelium-Like Phenotype In Vitro and the Dynamic Temporal Changes of Related Cytokines by Both Paracrine and Autocrine Signal Regulation
- Species:
- Human
- Sample Type:
- Conditioned Media (Adipose-derived Stem Cells)
- Year:
- 2014
- Journal Name:
- Plos One
SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGF Signals That Increase Expression of VEGF-C
- Species:
- Human
- Sample Type:
- Conditioned Media (Cervical Squamous Cell Carcinoma cells)
- Year:
- 2014
Higher propensity of Wharton's jelly derived mesenchymal stromal cells towards neuronal lineage in comparison to those derived from adipose and bone marrow.
- Species:
- Human
- Sample Type:
- Conditioned Media (WJ, BM and AD derived MSC w)
- Year:
- 2013
- Journal Name:
- Cell Biology International
- PubMed ID:
- 23418097
Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species.
- Species:
- Human
- Sample Type:
- Cell Lysate (adipose-derived stem cells UVB exposure)
- Year:
- 2013
- Journal Name:
- Stem Cells and Development
- PubMed ID:
- 22784094
Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Wharton's Jelly and BM derived MSCs)
- Year:
- 2012
- Journal Name:
- Cytotherapy
- PubMed ID:
- 22091833
A novel xenogeneic co-culture system to examine neuronal differentiation capability of various adult human stem cells.
- Species:
- Rat
- Sample Type:
- Conditioned Media (co-culturing of SCs with rat brain biopsies)
- Year:
- 2011
- Journal Name:
- Plos One
- PubMed ID:
- 21935488