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> Human IL-1 RA ELISA Kit

Human IL-1 RA ELISA Kit

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Cat# ELH-IL1ra-1

Size : 1PlateKit

Marca : RayBiotech


25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

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Human IL-1 RA ELISA Kit

Human IL-1 RA ELISA Kit

RayBio® Human IL-1 Ra (IL-1 F3) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
100 pg/ml

Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.1 ng/ml - 30 ng/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P18510
Gene Id
3557
Gene Symbols
IL1RN, IL1RA, IL1F3
Protein Name / Synonyms
Interleukin-1 receptor antagonist protein (IL-1RN) (IL-1ra) (IRAP) (ICIL-1RA) (IL1 inhibitor) (Anakinra)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human IL-1 RA ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IL-1Ra (IL-1RN / IL-1 F3 / IRAP / IL1RN), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

citations

  • GPRC5A/CXCL8/NLRP3-mediated neutrophil extracellular traps drive gemcitabine-nab-paclitaxel resistance in pancreatic adenocarcinoma

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants of GnP-resistant MIA PaCa-2 or PANC-1 cells)
    Year:
    2025
    Journal Name:
    Cancer Biology & Medicine
    PubMed ID:
    40665634

  • Sleep disturbances, inflammation, and muscle function in older adults with sarcopenia

    Species:
    Human
    Sample Type:
    Whole Blood (older adults with sarcopenia)
    Year:
    2026
    Journal Name:
    Sleep & breathing = Schlaf & Atmung
    PubMed ID:
    42696225

  • People with cystic fibrosis with high sputum neutrophil elastase on elexacaftor-tezacaftor-ivacaftor exhibit worse pulmonary function and pro-inflammatory airway milieu

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (sputum supernatants)
    Year:
    2025
    Journal Name:
    Scientific Reports
    PubMed ID:
    41361201

  • Sleep disturbances linked to plasma orexin A levels in patients with bipolar disorder: A preliminary study

    Species:
    Human
    Sample Type:
    Plasma (bipolar disorder patients)
    Year:
    2025
    Journal Name:
    Comprehensive psychoneuroendocrinology
    PubMed ID:
    41127685

  • Chondrocyte Response to Fresh Autologous Conditioned Serum Versus Freeze-Dried Allogenic Conditioned Serum

    Species:
    Human
    Sample Type:
    Other / Unspecified (cultured media)
    Year:
    2026
    Journal Name:
    Cartilage
    PubMed ID:
    39095949

  • Resistance Training Improves Sleep and Anti-Inflammatory Parameters in Sarcopenic Older Adults: A Randomized Controlled Trial

    Species:
    Human
    Sample Type:
    blood
    Year:
    2022
    Journal Name:
    International Journal of Environmental Research and Public Health

  • Urine protein biomarkers of bladder cancer arising from 16-plex antibody-based screens

    Species:
    Human
    Sample Type:
    Urine (bladder cancer patients and controls)
    Year:
    2021
    Journal Name:
    Oncotarget
    PubMed ID:
    33889301

  • Cell-free Stem Cell-Derived Extract Formulation for Regenerative Medicine Applications

    Species:
    Human
    Sample Type:
    Other / Unspecified (cell-free stem cell-derived extract CCM)
    Year:
    2020
    Journal Name:
    International Journal of Molecular Sciences
    PubMed ID:
    33316880

  • Steroids induce a disequilibrium of secreted interleukin-1 receptor antagonist and interleukin-1 synthesis by human neutrophils.

    Species:
    Human
    Sample Type:
    Conditioned Media (Neutrophils ); Plasma
    Year:
    2011
    PubMed ID:
    20650986

  • Does intestinal epithelial integrity status in response to high-protein dairy milk beverage with or without progressive resistance training impact systemic inflammatory responses in an active aging population?

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2022
    Journal Name:
    Plos One

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