Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

IgG (kappa chain specific) (Texas Red)

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Cat# I1903-37M-1mg

Size : 1mg

Marca : US Biological

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I1903-37M IgG (kappa chain specific) (Texas Red)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

F(ab’)2

Grade

Affinity Purified

Applications

FLISA IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in FLISA and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label:
Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons)

Absorption Wavelength: 596 nm

Emission Wavelength: 620 nm

Fluorochrome/Protein Ratio: 1.8 moles Texas Red per mole of Goat IgG F(ab’)2

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Texas Red conjugates are sensitive to light.

Applications

Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: human|Concentration: ~1mg/ml|Form: Supplied as a lyophilized powder from 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide|Purity: Purified by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |Immunogen: Human kappa light chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human kappa light chain

Form

Supplied as a lyophilized powder from 10mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide

Purity

Purified by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.