Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His)
Cat# NB-64-57277-20ug
Size : 20ug
Marca : Neo Biotech
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Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His)
(Synonyms: VLA5A, VLA-5, integrin, α5 (fibronectin receptor, α polypeptide), integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α5 β 1, Integrin alpha 5 β1, FNRA, CD49e) Copy Product InfoSynonyms: VLA5A, VLA-5, integrin, α5 (fibronectin receptor, α polypeptide), integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α5 β 1, Integrin alpha 5 β1, FNRA, CD49e
Catalog No. TMPY-02159 Copy Product Info
Purity: 95.7%
Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 192 kDa and the accession number is P08648&P05556-1.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:95.7%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human Integrin alpha 5 beta 1 Protein (Flag & His) (TMPY-02159) at 1 μg/mL (100 μL/well) can bind Volociximab (T9901A-008). The EC50 is 5.180 ng/mL. |
| Description | Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 192 kDa and the accession number is P08648&P05556-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-Flag, C-His |
| Accession Number | P08648&P05556-1 |
| Construction | A DNA sequence encoding the extracellular domain (Met 1-Tyr 995) of human ITGA5 (P08648) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified Predicted N terminal: Phe 42 & Gln 21 |
| Protein Purity | > 90 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | VLA5A, VLA-5, integrin, α5 (fibronectin receptor, α polypeptide), integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α5 β 1, Integrin alpha 5 β1, FNRA, CD49e |
| Molecular Weight | 192 kDa (predicted); 120 &140 kDa (reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Keywords
VLA5VLA 5integrin, α 5integrin, alpha 5integrin, a 5fibronectin receptor, α polypeptidefibronectin receptor, alpha polypeptidefibronectin receptor, a polypeptide
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Related Tags: Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) chemical structure | Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) molecular weight
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