KCC2 Antibody
Cat# OASE00240
Size : 100ug
Marca : Aviva Systems Biology
| Datasheets/Manuals | Printable datasheet for KCC2 Antibody (OASE00240) |
|---|---|
| COA Datasheet | 1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide. |
| Clonality | Monoclonal |
| Clone | S1-12 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | ICC,IF,IHC,IP,WB |
| Additional Information | Cellular Localization: Membrane |
| Reconstitution and Storage | -20°C |
| Immunogen | Fusion protein amino acids 932-1043 corresponding to rat KCC2 |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Specificity | Detects ~140kDa. |
| Dilution | WB (1:1000), IHC (1:300), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Application Info | WB: 1:1000 IHC: 1:300 ICC: 1:100 IF: 1:100 Optimal dilutions should be determined by the end user. |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Publications | GABAergic signaling promotes early-life seizures in epileptic SYNGAP1sup>+/-/sup> mice Preprint — no PubMed record available Targeting ischemia-induced KCC2 hypofunction rescues refractory neonatal seizures and mitigates epileptogenesis in a mouse model Preprint — no PubMed record available TrkB agonists prevent postischemic emergence of refractory neonatal seizures in mice |
| Description |
| Reference | 1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110. |
|---|---|
| Gene Symbol | Slc12a5 |
| Gene Full Name | solute carrier family 12 member 5 |
| Alias Symbols | electroneutral potassium-chloride cotransporter 2, furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, Kcc2, neuronal K-Cl cotransporter, neuronal-specific K-Cl cotransporter, solute carrier family 12 (potassium-chloride transporter), member 5, solute carrier family 12 member 5. |
| NCBI Gene Id | 171373 |
| Protein Name | Solute carrier family 12 member 5|solute carrier family 12 member 5 [Rattus norvegicus] |
| Description of Target | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (PubMed:9930699). Involved in the regulation of de
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