Size : 5mg
Synonyms: LDS751
| Description | LDS-751 is a cell-permeable long-wavelength nucleic acid fluorescent dye primarily used for DNA detection, cellular nucleic acid staining, and flow cytometry analysis, with significantly enhanced fluorescence upon binding to dsDNA. Under dsDNA conditions, typical Ex = 541 nm, Em = 708 nm. |
| In vitro | Method: Peripheral blood cells were stained with LDS-751 during flow cytometric sample preparation, and intact cells were discriminated from erythrocyte ghosts, platelets and damaged nucleated cells. Result: LDS-751 staining enabled identification of intact cells and separation of the major leukocyte populations using fluorescence and light-scatter characteristics.[1] Method: Fresh unfixed human peripheral blood was mixed with LDS-751 and fluorescent antibodies and analyzed by multiparameter flow cytometry. Result: Neutrophils, lymphocytes and monocytes were resolved using side scatter and LDS-751 fluorescence, while erythrocytes and platelets stained weakly.[2] Method: Viable nucleated cells were stained with LDS-751 and compared with established mitochondrial and nucleic-acid stains using fluorescence microscopy and flow cytometry. Result: LDS-751 predominantly stained mitochondria in viable cells, with staining behavior resembling the mitochondrial dye rhodamine 123 and decreasing after mitochondrial depolarization.[3] |
| Synonyms | LDS751 |
| Molecular Weight | 471.98 |
| Formula | C25H30ClN3O4 |
| Cas No. | 181885-68-7 |
| Smiles | O=Cl(=O)(=O)[O-].C(C=CC1=CC=C2C=C(C=CC2=[N+]1CC)N(C)C)=CC3=CC=C(C=C3)N(C)C |
| Relative Density. | no data available |
| Storage | Keep away from moisture,Keep away from direct sunlight,The compound is unstable in solution. Please use soon, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 66.64 mg/mL (141.19 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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