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GAL4 (FITC)

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Cat# G1016-50-FITC-100ul

Size : 100ul

Marca : US Biological


G1016-50-FITC GAL4 (FITC)

Clone Type
Monoclonal
Host
mouse
Isotype
IgG1,k
Grade
Affinity Purified
Applications
FLISA IC IF IP WB
Crossreactivity
Ye
Shipping Temp
Blue Ice
Storage Temp
-20°C

GAL4 protein is a transcriptional activator that is required for the expression of genes that encode enzymes of galactose utilization. The N-terminal domain of GAL4 binds to specific DNA sequences and the C-terminal domain contains acidic regions, which are required for activating transcription. GAL4 has been useful in two-hybrid assays for the study of protein-protein interactions.

Applications:
Suitable for use in Immunofluorescence, Immunocytochemistry, Immunoprecipitation, FLISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 3G152|Host: mouse|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography from ascites.|Immunogen: Recombinant protein corresponding to aa1-147 mapping within the N-terminal, DNA binding domain of GAL4.|Specificity: Recognizes GAL4. Specific for the DNA binding domain of the S. cerevisiae GAL4 protein. The immunoreactive epitope maps within aa1-92 of GAL4. Suitable for the detection of both N-terminal and C-terminal tagged GAL4 (1-147) and GAL4 (1-92) fusion proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to aa1-147 mapping within the N-terminal, DNA binding domain of GAL4.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes GAL4. Specific for the DNA binding domain of the S. cerevisiae GAL4 protein. The immunoreactive epitope maps within aa1-92 of GAL4. Suitable for the detection of both N-terminal and C-terminal tagged GAL4 (1-147) and GAL4 (1-92) fusion proteins.
References
1. Fields, S. & Song, O., Nature 340: 245-246 (1989). 2. Sadowski, I. & Ptashne, M., Nucleic Acids Res. 17: 7539 (1989).