>95% pure. Prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated below.
Buffer
State: Liquid (sterile filtered) Ig fraction. Buffer System: 0.1M Tris Chloride, 0.5M Sodium Chloride, pH 8.0 with 0.01% (w/v) Sodium Azide as preservative.
Preparation
Liquid (sterile filtered) Ig fraction.
Recommended Dilution
Suitable for use as antigen or ligand in Immunochemical Reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.
Protein Description
Purified polyclonal Mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (µ chain specific). No reaction was observed against anti-Mouse IgG F(c). Some light chain cross reactivity will occur with anti-Mouse IgG.
Storage
Store undiluted at 2-8°C for one month or (mix with an equal amount of glycerol and aliquot) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Stability
Shelf life: One year from despatch.
Shipping
Blue Ice
Reference Data
Synonyms
Immunoglobulin M mouse
Protein Families
as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins., Suitable for use as antigen or ligand in Immunochemical Reactions
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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