Mouse lambda light chain Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommended Dilution	ELISA: 1/10,000 - 1/50,000 . Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry on paraffin sections: 1/500 - 1/2,500.
Reactivities	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Mouse lambda light chain
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative Label: HRP State: Lyophilized purified Ig fraction Label: Horseradish Peroxidase
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed.
Conjugation	HRP
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.