Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Neutrophil Elastase (NE) Activity Colorimetric Assay Kit

Stampa

Cat# E-BC-K899-M-96T

Size : 96T

Marca : Elabscience

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Manual MSDS

Detection Principle	Neutrophil Elastase (NE) is one of the main serine proteases secreted by neutrophils. It plays an important role in the physiological and pathological processes of the body, on the one hand NE participate in the formation of the body's defense system and assist in the elimination of pathogenic microorganisms. On the other hand, the excessive activation of NE, in the case of imbalance with endogenous inhibitors, is directly involved in tissue damage, and is closely related to the occurrence of a variety of inflammation-related diseases, such as atherosclerosis, chronic obstructive pulmonary disease, pulmonary cystic fibrosis and other diseases, which can be used as a prevention and treatment target for these diseases. The detection principle of this kit is as follows: NE hydrolyzates the substrate to generate chromogenicity substances, which have maximum absorption at the wavelength of 380 nm, and NE activity in the sample is calculated by measuring the OD value at 380 nm and the standard curve.
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (370-390 nm)
Research Area	Amino Acids And Proteins
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.54 U/L
Detection Range	0.54-28.57 U/L
Precision	inter-assay CV: 6.3-7.5% \| intra-assay CV: 4.1-5.0%
Sample Volume	20 μL(tissue homogenate)
Assay Time	1 h 20 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse Pancreas tissue homogenate	5-15
10% Mouse Small intestine tissue homogenate	1
10% Mouse Stomach tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.