Polyclonal Goat Anti-Human IgM Secondary Antibody (AP, IHC, WB)

Human IgM

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE, FITC, Biotin, HRP, Rhodamine, FITC.

Immunoaffinity purified

Unmodified

Human IgM mu chain.

Recognizes human IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-goat serum, human IgM and human serum. No reaction was observed against human IgG F(c). Specificity was confirmed by ELISA at less than 1% cross-reactivity against other human heavy or light chain isotypes.

Suitable for use in ELISA, Western or Dot Blot and Immunohistochemistry. ELISA: 1:1000-1:3000. Western Blot: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesium chloride, 0.1 mM zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.

Store at 4°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.