Rat Histone Deacetylase 1, HDAC1 ELISA Kit

Cat# E0854Ra-96wells

Size : 96wells

Marca : Bioassay Technology Laboratory

Contatta il distributore locale :


Telefono : +1 850 650 7790

Rat Histone Deacetylase 1, HDAC1 ELISA Kit

Rat Histone Deacetylase 1, HDAC1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.096ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.096ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Histone Deacetylase 1

Code

E0854Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.096ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.

UniProt accession

MASS(Da)

55,093

GeneID

297893

Synonyms

HD1;HDAC 1;HDAC1;Histone deacetylase 1

Gene names

HDAC1

Research area

Others

Target protein

HDAC1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0854Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 1.908 2.012 1.97
2.116
20ng/ml 0.979 0.961 0.919
0.943
10ng/ml 0.517 0.533 0.491
0.548
5ng/ml 0.275 0.295 0.253
0.315
2.5ng/ml 0.095 0.122 0.08
0.149
0ng/ml 0.026 0.042 0
0.058

Precision

We measured random samples of E0854Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 21.9 0.81 3.7
Intra-Assay 2 18 32.9 0.88 2.7
Intra-Assay 3 18 9.9 0.53 5.3

Protocols

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