Rat Histone Deacetylase 1, HDAC1 ELISA Kit
Cat# E0854Ra-96wells
Size : 96wells
Marca : Bioassay Technology Laboratory
Product overview
Full product name
Histone Deacetylase 1
Code
E0854Ra
Assay type
Sandwich
Size
48T,96T
Sensitivity
0.096ng/ml
Detection range
0.2-60ng/ml
Sample type
Serum, plasma, cell culture supernates
Species
Rat
Storage
2-8ºC
Assay time
1h 30m
Background
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
UniProt accession
MASS(Da)
55,093
GeneID
297893
Synonyms
HD1;HDAC 1;HDAC1;Histone deacetylase 1
Gene names
HDAC1
Research area
Others
Target protein
HDAC1
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0854Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
40ng/ml | 1.908 | 2.012 | 1.97 |
2.116 | |||
20ng/ml | 0.979 | 0.961 | 0.919 |
0.943 | |||
10ng/ml | 0.517 | 0.533 | 0.491 |
0.548 | |||
5ng/ml | 0.275 | 0.295 | 0.253 |
0.315 | |||
2.5ng/ml | 0.095 | 0.122 | 0.08 |
0.149 | |||
0ng/ml | 0.026 | 0.042 | 0 |
0.058 |
Precision
We measured random samples of E0854Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 21.9 | 0.81 | 3.7 |
Intra-Assay | 2 | 18 | 32.9 | 0.88 | 2.7 |
Intra-Assay | 3 | 18 | 9.9 | 0.53 | 5.3 |
Protocols
Customer reviews and Q&As