Rat IgG1 Isotype Control
Cat# MBS8322374-0,1mL
Size : 0.1mL
Marca : MyBiosource
IgG1 Isotype Control – Rat Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8322374Rat IgG1 kappa Isotype Control can be used as an isotype control in flow cytometry and other applications. More details
Product Overview
Online Datasheet in PDFProduct Name
IgG1, Isotype Control
Full Product Name
Rat IgG1 Isotype Control
Product Gene Name
IgG1 isotype control
Clonality
Monoclonal
Isotype
Rat IgG1
Clone Number
GL113
Host
Rat
Form/Format
Rat IgG1. Liquid in PBS, pH7.3, and 0.02% sodium azide.
Purity/Purification
The antibody was purified by affinity chromatography.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Directions for Use
1. Take 100 ul peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
Product Categories/Family for IgG1 isotype control
Primary Antibodies
Applications Tested/Suitable for IgG1 isotype control
Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Other Notes
Small volumes of IgG1 isotype control vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IgG1 purchase
MBS8322374 is an isotype control targeting IgG1. Also known as Rat IgG1 Isotype Control. Host: Rat.
Additional Details
NCBI/Uniprot data below describe general gene information for IgG1. It may not necessarily be applicable to this product.
Protein Family
Interleukin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.