Recombinant Mouse IFN alpha2 (E.coli)

Biological Activity

Recombinant mouse interferon Alpha-2 is produced by an E. coli expression system where the target gene encoding Cys24-Glu190 is expressed. There are at least 23 different variants of interferon-alpha known. The molecular mass of each protein ranges from 19-26 kD and consists of proteins of 156-166 and 172 amino acids in length. All IFN-α isoforms share a common conserved sequence region between amino acid positions 115-151, while the amino acid terminus is variable. Many IFN-α isoforms have sequence differences at only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl terminus.

Accession: P01573

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 16 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 6% Sucrose, 4% Mannitol, 0.02% Tween80 (w/v), pH 6.0.

Chemical Information

Form	Lyophilized powder
Solubility (25°C)	Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Biswas Neupane, et al. Front Immunol. Interleukin-17A Facilitates Chikungunya Virus Infection by Inhibiting IFN-α2 Expression

[2] Jieliang Chen, et al. Hepatology. Functional Comparison of Interferon-α Subtypes Reveals Potent Hepatitis B Virus Suppression by a Concerted Action of Interferon-α and Interferon-γ Signaling

[3] George Avraamides, et al. J Interferon Cytokine Res. IFN-alpha2 induces leukocyte integrin redistribution, increased adhesion, and migration

[4] L Santodonato, et al. Cancer Gene Ther. Antitumor activity of recombinant adenoviral vectors expressing murine IFN-alpha in mice injected with metastatic IFN-resistant tumor cells

[5] G C Viscomi, et al. J Interferon Cytokine Res. Antigenic characterization of recombinant, lymphoblastoid, and leukocyte IFN-alpha by monoclonal antibodies