RH FGF-6
Cat# 100-201-1MG
Size : 1MG
Marca : FUJIFILM
Recombinant Human FGF-6
Fibroblast growth factor 6 (FGF-6) is a heparin-binding growth factor that is expressed in epithelial and mesenchymal lineages. FGF-6 binds and signals through the FGF receptors FGFR1, FGFR2, and FGFR4. FGF-6 functions as a mitogen for vascular endothelial cells and fibroblasts. FGF-6 is also an important factor driving muscle differentiation and regeneration.
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Cell based proliferation assay
Figure 1. Recombinant Human FGF-6 induced proliferation in NR6R/3T3 cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Heparin secretory-transforming protein 2, HST-2, HSTF-2, Heparin-binding growth factor 6, HBGF-6 |
| Accession Number | P10767 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 18.9 kDa (169 aa) |
| AA Sequence | MGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | NR6R-3T3 Proliferation with 1 µg heparin | ≤ 1 ng/mL; ≥ 1.0 x 106 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.