TET1 ELISA Kit (Human)
Cat# OKEH08049
Size : 96T
Marca : Aviva Systems Biology
TET1 ELISA Kit (Human) (OKEH08049)
| Datasheets/Manuals | Printable datasheet for TET1 ELISA Kit (Human) (OKEH08049) |
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| COA Datasheet | Printable COA datasheet for OKEH08049 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology TET1 ELISA Kit (Human) (OKEH08049) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TET1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TET1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TET1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=6.7% (n = 20) Mean Inter-assay CV%: <=7.3% (n = 20) | ||||||||||||||||||||||
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| Additional Information | Function: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789). | ||||||||||||||||||||||
| :: | Subcellular Location: Nucleus | ||||||||||||||||||||||
| :: | Unigene: Hs.258855 SMR: Q8NFU7 String: 9606.ENSP00000362748 | ||||||||||||||||||||||
| :: | KEGG: hsa:80312 MIM: 607790 Pfam: PF12851 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 0.069ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Methylcytosine dioxygenase TET1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | TET1 |
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| Gene Full Name | tet methylcytosine dioxygenase 1 |
| Alias Symbols | bA119F7.1, CXXC finger 6, CXXC zinc finger 6, CXXC-type zinc finger protein 6, CXXC6, LCX, leukemia-associated protein with a CXXC domain, methylcytosine dioxygenase TET1, ten-eleven translocation 1 gene protein, ten-eleven translocation-1, tet oncogene 1, TET1 splice variant VP_DE4, TET1 splice variant VP_DE456. |
| NCBI Gene Id | 80312 |
| Protein Name | Methylcytosine dioxygenase TET1 |
| Description of Target | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789). |
| Uniprot ID | Q8NFU7 |
| Protein Accession # | NP_085128.2 |
| Nucleotide Accession # | NM_030625.2 |