Trypsin Reagent
Cat# MBS258487-15mL
Size : 15mL
Marca : MyBiosource
Trypsin Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258487Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane… More details
Product Overview
Online Datasheet in PDFProduct Name
Trypsin, Reagent
Full Product Name
Trypsin Reagent
Preparation and Storage
Store at 2-8 degree C. DO NOT FREEZE
Kit Contents
Reagent B: (Buffer) 15 ml
Reagent E: (Enzyme concentrated), 2ml
Reagent E: (Enzyme concentrated), 2ml
Notes
This enzyme is supplied as concentrated form along with buffer
Product Categories/Family for Trypsin reagent
IHC Reagents
Applications Tested/Suitable for Trypsin reagent
Immunohistochemistry (IHC)
Application Notes for Trypsin reagent
1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or Universal Immuno buffer. Add endogenous peroxidase block as usual. Wash tissue section 3-5 times with buffer.
2. Remove buffer, add 2-4 drops of ready-to use enzyme solution to cover the tissue section.
3. Incubate at 37 degree C for 5-10 minutes or 10-15 minutes at room temperature, (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-15 minutes are sufficient at the above temperatures. In some cases it may require more time).
4. Discard trypsin reagent, wash 3-5 times with buffer.
5. The tissue sections are ready for further IHC protocol.
2. Remove buffer, add 2-4 drops of ready-to use enzyme solution to cover the tissue section.
3. Incubate at 37 degree C for 5-10 minutes or 10-15 minutes at room temperature, (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-15 minutes are sufficient at the above temperatures. In some cases it may require more time).
4. Discard trypsin reagent, wash 3-5 times with buffer.
5. The tissue sections are ready for further IHC protocol.
Other Notes
Small volumes of Trypsin reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Trypsin purchase
MBS258487 is a reagent targeting Trypsin.
Additional Details
NCBI/Uniprot data below describe general gene information for Trypsin. It may not necessarily be applicable to this product.
Protein Family
Trypsin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.