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> VAChT Antibody

VAChT Antibody

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Cat# ASM10227-P594-100

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Marca : Abcepta

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  • ICC/IF - VAChT Antibody ASM10227
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (ASM10227). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (ASM10227) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VAChT Antibody (D) Composite.
  • WB - VAChT Antibody ASM10227
    Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (ASM10227). Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (ASM10227) at 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession Q16572
Other Accession NP_003046.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human VAChT Monoclonal IgG1
Target/Specificity Detects ~56kDa.
Other Names Vesicular Acetylcholine Transporter Antibody, MGC12716 Antibody, rVAT Antibody, Slc18a3 Antibody, Solute carrier family 18 (vesicular acetylcholine) member 3 Antibody, Solute carrier family 18 (vesicular monoamine) member 3 Antibody, Solute carrier family 18 member 3 Antibody
Clone Names S6-38
Immunogen Synthetic peptide amino acids 521-532 of human VAChT
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A dilution of 1:50-1:200 of SMC-341 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane