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> Citrate BioAssay™ Kit

Citrate BioAssay™ Kit

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Cat# 172366-100T

Size : 100Tests

Marca : US Biological

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172366 Citrate BioAssay™ Kit

Clone Type
Polyclonal
Shipping Temp
Dry Ice
Storage Temp
-20°C

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570nm or fluorescence intensity at λex/em=530/585nm is directly proportional to the citrate concentration in the sample.

Intended Use:
For citrate determination in biological samples (e.g. serum, plasma, urine, tissue and culture media).

Key Features:
Fast and sensitive: Linear detection range: 4 to 400uM citrate for
colorimetric assays and 0.5 to 40uM for fluorimetric assays.

Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling
systems for processing thousands of samples per day.

Kit Components:
172366A: Developer, 1x10ml
172366B: Dye Reagent, 1x120ul
172366C: Citrate Standard, 1x500ul
172366D: CL Enzyme, 1x1vial
172366E: ODC Enzyme, 1x120ul

Storage and Stability:
Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Pant, A. et al (2021). Viral growth factor-and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection. PLoS Pathogens, 17(2). 2. Fu, X., et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. 3. Trivedi, A. K., et al. (2015). Adaptation of oxidative phosphorylation to|photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184: 34-40.